Fig. 1

H3F3A mutations and patients characteristics of the study group. a Chromatograms showing the H3F3A sequence spanning codon 27 and 34 illustrate the wild-type status and mutations that exchange either the amino acid lysine with methionine (K27M), glycin (G34) with tryptophan (G34W) or with arginine (G34R) b Charts show the overall incidence of H3G3A G34W mutations in our series of osteosarcomas over all age groups and in the group of patients older than 30 years. c Osteosarcomas are separated in wild-type and mutant regarding their H3F3A status. Giant cell tumors of bone act as control. The age distribution is demonstrated for each case. The black bar indicates the median age. Gender distribution is illustrated in the form of a diagram. The site of occurrence is only shown for cases that were further analysed for methylome and copy number profiling. Each ring/dot represents a case. Red color codes for cases that were further analyzed for methylome and copy number profiling