Table 4 Experimental conditions tested
Test | Experimental conditions |
|---|---|
Steroids | E2 (Fig 2A), ASD, DHT (100 pM-1 μM) |
Inhibitors of estrogen signaling | OHT, Fulvestrant, Anastrozole, Letrozole, Exemestane (1 nM-10 μM) |
Steroids combined with inhibitors | E2 (1 nM) with OHT or Fulvestrant (1 nM-10 μM) (Fig. 2B) |
FBS | 1%, 5%, 10% FBS alone and combined with E2 or ASD (100 pM-1 μM) |
Timepoints of measurements | 3 days, 7 days |
Methods of measurement | WST1 viability assay, cell counting |