Figure 5

Flow cytometry and purity testing of sorted fractions. (A) Viable, single, human (TRA-1-85+) SW872 xenograft-derived cells (98, 8%) were sorted on the basis of (B) Aldefluor (X-axis) and CD133 (Y-axis) activity. In this representative experiment the subpopulations in the culture were as follows: 79% Aldefluor^low CD133^low, 6% Aldefluor^low CD133^high, 14% Aldefluor^high CD133^low and 0, 9% Aldefluor^high CD133^high. The 4 flow sorted subpopulations were subject to subsequent purity testing: (C) Aldefluor^high CD133^high: 33% pure, (D) Aldefluor^high CD133^low: 71% pure and containing 0, 3% potential CSCs (E) Aldefluor^low CD133^high: 55% pure and (F) Aldefluor^low CD133^low: 96% pure.