Liposarcoma cells with aldefluor and CD133 activity have a cancer stem cell potential

Table 1 CD133 and ALDH1 expression in liposarcoma patient samples.

Diagnosis	Tumour site	Age	Pre-treatment	Grade	CD133	ALDH1
						Cytoplasm	Nucleus
Well-diff.	Retroperitoneal	36	No treatment	1	0	3	3
Well-diff.	Retroperitoneal	57	No treatment	2	0	3	3
Myx/roundcell	Thigh	41	No treatment	3	0	3	3
Myx/roundcell	Thigh	79	Chemotherapy	4	0	3	3
De-diff.	Thigh	79	No treatment	4	0	3	3
De-diff.	Retroperitoneal	60	No treatment	4	0	1	1
Well-diff. Comp *	Retroperitoneal	64	No treatment		0	3	3
De-diff. Comp*	Retroperitoneal	64	No treatment	4	0	3	3
Pleomorphic	Truncus	67	No treatment	4	0	3	3
Pleomorphic	Retroperitoneal	58	No treatment	4	0	3	2
Well-diff.	Leg	31	No treatment	1	0	2	2

Ten liposarcomas diagnosed as well-differentiated (well-diff.), myxoid/roundcelled (myx/roundcell), de-differenitated (de-diff) or pleomorphic were included in the analyses. Tumour location, patient age, treatment prior to sample collection and tumour grade are also displayed. CD133 and ALDH1 expression was scored as follows: 0 = negative, 1 = less than 10% of the tumour cells scored positive, 2 = 10-50% of the rumour cells scored positive, 3 = more than 50% of the tumour cells scored positive. *For one of the tumors, a de-differentiated and a well-differentiated component was analysed.

ISSN: 2045-3329