Liposarcoma cells with aldefluor and CD133 activity have a cancer stem cell potential

Table 2 Phenotypic analyses of SW872.

SW872	Xenograft-derived cells	Xenograft-derived cells	Cell line
Surface marker	SC-medium	RPMI	RPMI
FGFR-1^high	86, 8	62, 8	43, 4
Aldefluour^high	35, 0	11, 0	0, 2
CD90^high	93, 3	89, 4	41, 6
CD44^high	97, 9	98, 3	99, 9
CD105^high	97, 5	95, 6	82, 1
STRO-1^high	0, 5	0, 7	0, 3
CD73^high	2, 6	4, 4	27, 4
CD133^high	0, 6	0, 3	0, 3
Aldefluour^high CD90^high	41, 9	5, 5	ND
Aldefluour^high CD44^high	39, 8	3, 7	ND
Aldefluour^high CD105^high	44, 8	2, 8	ND
Aldefluour^high STRO-1^high	0, 2	0, 1	ND
Aldefluour^high CD73^high	1, 3	3, 2	ND
Aldefluour^high CD133^high	0, 1	0	ND

The table displays the average percentages of cells scored as Aldelfuor^high, surface marker^high or Aldelfuor^high surface marker^high in the respective culturing medium, as determined by flow cytometry (minimum two parallels were performed). The SW872 cell line was not subjected to co-staining as only 0, 2% of the cells were scored as Aldefluor^high.

ISSN: 2045-3329