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Figure 1 | Clinical Sarcoma Research

Figure 1

From: The CXCR4-CXCL12 axis in Ewing sarcoma: promotion of tumor growth rather than metastatic disease

Figure 1

Immunohistochemical analysis of expression of the CXCR4-CXCL12 axis in Ewing sarcoma. A F. Light micrographs (20x magnification) of immunohistochemical stainings for CXCR4 and CXCL12 expression in therapy-naive and metastatic EWS. Representative examples. A-C: strong (++), moderate (+) and absent (−) CXCR4 expression, respectively. D-F: strong (++), moderate (+) and weak (+/−) CXCL12 expression, respectively. Whereas CXCR4 was expressed solely by tumor cells, CXCL12 was observed in both tumor and stromal areas. Insets: immunoreactivity for both CXCR4 and CXCL12 was exclusively localized in the cytoplasm of cells. Intensity of staining was (semi-quantitatively) scored according to the quality control system as proposed by Ruiter et al. (see Methods section)[19]. (Decalcified) tonsil tissue sections were used for initial optimization of immunohistochemical staining procedures.

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