Skip to main content
Figure 1 | Clinical Sarcoma Research

Figure 1

From: Histone deacetylase inhibitors enhance expression of NKG2D ligands in Ewing sarcoma and sensitize for natural killer cell-mediated cytolysis

Figure 1

Chemotherapy-resistant Ewing sarcoma display significantly reduced susceptibility to lysis by resting natural killer cells. A-B. Cytotoxic activity of resting natural killer cells was evaluated in 51Cr release assays using chemotherapy-sensitive (grey) and -resistant (black) Ewing sarcoma cell lines TC71 (grey dots), SK-ES-1 (grey squares) and SK-N-MC (grey triangles) respectively STA-ET2.1 (black squares), CADO-ES (black dots) and IOR/BER (black triangles) as target cells. Classical natural killer cell target K562 and EBV B-LCL cell line 107 were used as positive and negative control respectively (A). Results are expressed as the mean ± SEM percentage of specific lysis obtained in at least four independent experiments using different healthy donors. Statistical analysis (t-test) was performed on mean percentages of specific lysis of chemotherapy-sensitive versus -resistant cell lines, revealing significantly reduced sensitivity of chemotherapy-resistant cell lines to resting natural killer cell-mediated cytolysis at all effector-to-target ratio's ≥ 2.5:1 (p < 0.05) (B).

Back to article page