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Figure 2 | Clinical Sarcoma Research

Figure 2

From: Histone deacetylase inhibitors enhance expression of NKG2D ligands in Ewing sarcoma and sensitize for natural killer cell-mediated cytolysis

Figure 2

Non-differential expression of natural killer cell receptor ligands among chemotherapy-sensitive and -resistant Ewing sarcoma in vitro or in vivo. A. Constitutive surface expression of inhibitory (HLA class I) or activating (MICA/B, ULBP1-3, CD112, CD155) natural killer cell receptor ligands in chemotherapy-sensitive (grey; TC71, SK-ES-1 and SK-N-MC) and -resistant (black; CADO-ES, STA-ET2.1 and IOR/BER) Ewing sarcoma cell lines, as assessed by flow cytometry. Results are expressed as the mean ± SD MFI-ratio, obtained in at least two independent experiments. B. Representative examples of flow cytometry plots for HLA class I, MICB and ULBP-2 for several Ewing sarcoma cell lines; isotype matched control staining is shown in grey. C-D. Light micrographs (20 × magnification) of immunohistochemical stainings for MICA (c; strong expression) and ULBP-1 (d; moderate expression) in sequential primary Ewing sarcoma tumours. E. ELISA for detection of soluble MICA in plasma samples obtained from Ewing sarcoma patients (either prior to start of chemotherapy ('pre-treatment') or after completion of therapy ('post-treatment')) and age-comparable controls ('controls'). Statistical analysis (one-way ANOVA) revealed no significant differences in mean soluble MICA levels among 'controls' (39.63 pg/ml), 'pre-treatment' (17.67 pg/ml) or 'post-treatment' (25.14 pg/ml) patients (p > 0.05).

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