Table 1 EGFR oligonucleotide sequences for PCR analysis
Exon | Specific primers | PCR product |
|---|---|---|
Exon 18 | F : 5′- GCT GAG GTG ACC CTT GTC TC -3′ | 225 bp |
R: 5′- CTC CCC ACC AGA CCA TGA -3′ | ||
Exon 19 | F: 5′- CAT GTG GCA CCA TCT CAC A -3′ | 230 bp |
R: 5′- CAG CTG CCA GAC ATG AGA A -3′ | ||
Exon 20 | F: 5′- CAT TCA TGC GTC TTC ACC TG -3′ | 377 bp |
R: 5′- CAT ATC CCC ATG GCA AAC TC -3′ | ||
Exon 21 | F: 5′- GCT CAG AGC CTG GCA TGA A -3′ | 348 bp |
R: 5′- CAT CCT CCC CTG CAT GTG T -3′ |