Table 1 Monoclonal antibodies used in this study
From: Dendritic and mast cell involvement in the inflammatory response to primary malignant bone tumours
Antigen | Antibody (clone) | Specificity | Source | Dilution | Pre-treatment |
|---|---|---|---|---|---|
CD3 | (F7.2.38) | T cells | Dako | 1:25 | MW 20Â min |
CD11c | (5D11) | DCs, histiocytes | Novocastra | 1:100 | MW 20Â min |
CD14 | (7) | Macrophages, monocytes | Novocastra | 1:50 | MW 20Â min |
CD20 | (L26) | B cells | Dako | 1:100 | ─ |
CD45 | (PD7/26;2B11) | Leucocyte common antigen | Dako | 1:100 | MW 10Â min |
CD68 | (KP1) | Macrophages, monocytes, neutrophils, NK cells | Dako | 1:1000 | MW 10Â min |
DC-SIGN | (120,507) | Immature DCs | R&D Systems | 1:500 | MW 10Â min |
S100 | (polyclonal) | DCs, histiocytes, chondrocytes | Dako | 1:1000 | ─ |
Mast cell tryptase | AA1 | Mast cells | Dako | 1:100 | MW 10Â min |