TableĀ 2 Details of monoclonal and polyclonal antibodies employed for immunohistochemical staining of BAT and hibernoma
From: Immunophenotypic expression of UCP1 in hibernoma and other adipose/non adipose soft tissue tumours
Antibody (source) | Dilution | Antigen | Immunohistochemical staining |
|---|---|---|---|
1A4 (Agilent, UK) | 1/50 | Smooth muscle actin (SMA) | ā |
HHF 35 (Agilent, UK) | 1/50 | Muscle specific actin | ā |
DE-R11 (Agilent, UK) | 1/100 | Desmin | ā |
h-CD (Agilent, UK) | 1/200 | Caldesmon | ā |
CALP (Agilent, UK) | 1/50 | Calponin | + |
JC70 (Agilent, UK) | 1/20 | CD31 | + |
QBEnd 10 (Agilent, UK) | 1/50 | CD34 | ā |
PD7/26 (Agilent, UK) | 1/50 | CD45 | ā |
KP1 (Agilent, UK) | 1/1000 | CD68 | ā |
Anti S100a (Agilent, UK) | 1/50 | S100 | ++ |
Anti-FABP4 (Sigma, UK) | 1/2500 | aP2/FABP4 | ++ |
Anti-MIC2 (127E) (Leica, UK) | 1/50 | CD99 | ā |
AE1/3 (Leica, UK) | 1/50 | Pan-cytokeratin | ā |
E29 (Aligent, UK) | 1/500 | Epithelial membrane antigen | ā |
A103 (Aligent, UK) | 1/50 | Melan A | ā |
D2-40 (Aligent, UK) | 1/100 | Podoplanin | ā |
Ab10983 (Abicam, UK) | 1/250ā1/20,000a | UCP1 | ++ |