Table 2 Details of monoclonal and polyclonal antibodies employed for immunohistochemical staining of BAT and hibernoma
From: Immunophenotypic expression of UCP1 in hibernoma and other adipose/non adipose soft tissue tumours
| Antibody (source) | Dilution | Antigen | Immunohistochemical staining |
|---|---|---|---|
| 1A4 (Agilent, UK) | 1/50 | Smooth muscle actin (SMA) | − |
| HHF 35 (Agilent, UK) | 1/50 | Muscle specific actin | − |
| DE-R11 (Agilent, UK) | 1/100 | Desmin | − |
| h-CD (Agilent, UK) | 1/200 | Caldesmon | − |
| CALP (Agilent, UK) | 1/50 | Calponin | + |
| JC70 (Agilent, UK) | 1/20 | CD31 | + |
| QBEnd 10 (Agilent, UK) | 1/50 | CD34 | − |
| PD7/26 (Agilent, UK) | 1/50 | CD45 | − |
| KP1 (Agilent, UK) | 1/1000 | CD68 | − |
| Anti S100a (Agilent, UK) | 1/50 | S100 | ++ |
| Anti-FABP4 (Sigma, UK) | 1/2500 | aP2/FABP4 | ++ |
| Anti-MIC2 (127E) (Leica, UK) | 1/50 | CD99 | − |
| AE1/3 (Leica, UK) | 1/50 | Pan-cytokeratin | − |
| E29 (Aligent, UK) | 1/500 | Epithelial membrane antigen | − |
| A103 (Aligent, UK) | 1/50 | Melan A | − |
| D2-40 (Aligent, UK) | 1/100 | Podoplanin | − |
| Ab10983 (Abicam, UK) | 1/250–1/20,000a | UCP1 | ++ |