Fig. 2

Expression of lipid metabolic genes is progressively downregulated with dedifferentiation. a Gene expression ΔCt values of the 3M panel consisting of LIPE, PNPLA2 and PLIN1 are shown for each patient individually as bee swarm plots from healthy tissue, lipoma, WDLPS and DDLPS. Black horizontal lines indicate the median values in each group. Red dots annotate samples extracted from the WD component of tumors from patients with a DDLPS diagnosis. Two of the specimens were acquired from the same patient diagnosed with DDLPS, and corresponds to WD (blue) and DD (green) components. b Patient-wise hierarchical clustering heatmap of normalized expression levels of LIPE, PNPLA2 and PLIN1 (3M) across adipose controls, lipomas, WDLPS, DDLPS and WD components from DDLPS tumors. Red colors correspond to lowered ΔCt-values (higher expression), and blue corresponds to higher ΔCt values (lower expression). Patients were annotated based on their histological diagnosis. AT adipose tissue. HAT healthy adipose tissue, DDLPS = dedifferentiated liposarcoma, M18 metastasis positive patient (the arrow), ns non-significant, WDLPS well-differentiated liposarcoma