Expression of aromatase and estrogen receptor alpha in chondrosarcoma, but no beneficial effect of inhibiting estrogen signaling both in vitro and in vivo

Table 2 Procedures and details of the primary antibodies used for immunohistochemistry

Protein	Origin	Number	Dilution	Species	Antigen retrieval	Blocking	Positive control
ESR1	Invitrogen/zymed	18-0174Z	1:200	Rabbit	Tris-EDTA	30' 5% ELK milk	breast cancer
aromatase	Abcam	Ab18995	1:300	Rabbit	Citrate	30' 5% ELK milk	placenta
AR	Dako	AR441	1:200	Mouse	Tris-EDTA	-	cervix stroma

ISSN: 2045-3329