Characterisation of SW872 xenograft-derived cells following culturing in RPMI or stem cell medium. (A) Different morpholgy was observed dependent on the culturing medium. The cells appeared adherent when cultured in standard RPMI supplemented with fetal bovine serum (upper panel) and grew as detached spheroids when cultured in SC-medium (lower panel). (B) Flow diagrams are shown for control (DEAB) samples (left), and Aldefluor sample (right). 26% of the cells displayed Aldefluor activity when maintained in SC-medium (lower panel), compared to 13% of the cells when maintained in RPMI (upper panel). Aldefluor intensity is displayed along the X-axis. (C) Average Aldefluorhigh cells following culturing in SC-medium (35%) (black) (n = 10) or RPMI (11%) (grey) (n = 3).